Bird Flu virus has spread to two more regions including Barpeta and Nalbari in lower Assam.
The fresh outbreak prompted the State Veterinary department to start the culling of thousands of chickens in these regions.
The samples, which were sent to laboratories in Bhopal, have tested positive for H5N1 avian influenza.
The State Government has ordered culling operations and Rapid Response Teams (RRT)s have already begun going from house-to-house asking the habitants to give away their poultry birds to them.